.eight b (M12 124) seventeen.three (M12 122) 17.9 (M12 122) 45.2 (M12 431) 22.seven (M12 410) 38.eight (M12 197) 31.two (M12 173) 27.1 (M12 384) 46.0 (M12 381) 10.four (M

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Another rationalization is usually that potentially OmpA will not be Predicted purpose or domain ATPase Hypothetical E capsid. Another rationalization is usually that potentially OmpA will not be protein Putative glycanase/laminarinase Predicted tail fiber protein Predicted tail fiber assembly protein Predicted tail fiber assembly protein Hypothetical protein Predicted glycoprotein 5= nucleotidase, deoxy (pyrimidine), cytosolic kind C protein (NT5C) Hypothetical protein Hypothetical protein Hypothetical protein, DUF3820 superfamily Hypothetical protein Hypothetical proteinOtherM9 ORFP10VF 47.0 (P10VF_146)Cr19.nine (M9_134) 36.six (M9_138) 36.six (M9_137)thirty.two (P10VF_049), 17.9 (P10VF_051)36.9 (P10VF_039) 38.two (P10VF_004) 30.9 (P10VF_252) 58.six (P10VF_233) forty nine.2 (P10VF_231) eighteen.8 (P10VF_231)21.8.one (M9_216) eight.1 (M9_213)Not together with T4 main proteins or homing endonucleases. A person on the most stunning functions of your M9 and P10VF genomes could be the lack of a homolog for T4 gp33, which encodes the late transcription accent factor that acts in concert with gp55 to completely activate transcription as opposed to a bacterial RNA polymerase sigma component (62, 63). These phage RpoE proteins encoded by M9 and P10VF are candidates for late transcription sigma elements that might operate rather than gp55/gp33. The M9 genome also lacks a homolog for another member in the T4 core set of genes, the translational repressor encoded via the regA gene (fifty five)..eight b (M12_124) seventeen.3 (M12_122) 17.nine (M12_122) 45.two (M12_431) 22.seven (M12_410) 38.8 (M12_197) 31.2 (M12_173) 27.1 (M12_384) 46.0 (M12_381) 10.four (M12_381) 26.8 (M12_235) Comparable to ORFs in other phages of rhizobiaM9 ORF PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27463369 M9_035 M9_038 M9_121 M9_136 M9_137 M9_138 M9_141 M9_149 M9_176 M9_179 M9_210 M9_213 M9_216 M9_a bPredicted perform or area ATPase Hypothetical protein Putative glycanase/laminarinase Predicted tail fiber protein Predicted tail fiber assembly protein Predicted tail fiber assembly protein Hypothetical protein Predicted glycoprotein 5= nucleotidase, deoxy (pyrimidine), cytosolic type C protein (NT5C) Hypothetical protein Hypothetical protein Hypothetical protein, DUF3820 superfamily Hypothetical protein Hypothetical proteinOtherM9 ORFP10VF forty seven.0 (P10VF_146)Cr19.9 (M9_134) 36.six (M9_138) 36.6 (M9_137)30.2 (P10VF_049), 17.9 (P10VF_051)36.nine (P10VF_039) 38.two (P10VF_004) 30.9 (P10VF_252) 58.6 (P10VF_233) forty nine.two (P10VF_231) 18.8 (P10VF_231)21.8.1 (M9_216) eight.one (M9_213)Not like T4 core proteins or homing endonucleases. See Table S4 in the supplemental material for sequence accession numbers. Proteins for which the M12 homolog was detected from the proteome (ten). c Homologs of all ORFs are found in M7 (GenBank accession no. KR052480) and M19 (GenBank accession no. KR052481). Homologs of all ORFs besides M9_210 are uncovered in N3 (GenBank accession no. KR052482).lobacter phage Cr30 materials essential information that clarifies the relatedness of the phages on this department in the tree. A person on the most shocking features of your M9 and P10VF genomes will be the lack of a homolog for T4 gp33, which encodes the late transcription accent aspect that acts in concert with gp55 to fully activate transcription in lieu of a bacterial RNA polymerase sigma variable (62, sixty three). In phage T4, the sigma70 area 4-like activity of gp33 is vital for total activation of late transcription and for creation of phage particles (64, 65).